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. 2020 Sep 24;13:9455–9463. doi: 10.2147/OTT.S259853

Figure 1.

Figure 1

Exendin-4 inhibits the activation and function of PSCs. (A–C) PSCs were treated with different doses of exendin-4 for 48 h. (A) mRNA expression of α-SMA and Coll-I determined using qRT-PCR (*P<0.05, **P<0.01, by Student’s t-test vs control). (B) Protein expression of α-SMA and Coll-I detected by Western blot. (C) Relative quantitative comparison of Western blot analysis (*P<0.05, **P<0.01, by Student’s t-test vs control). (D) Immunofluorescence staining of α-SMA and Coll-I in PSCs after treatment with 50 nM exendin-4. Representative images are shown at 300× magnification. Scale bar: 50 μm (E) Cell viability of PSCs treated with or without exendin-4 determined by CCK-8 assay (*P<0.05, by Student’s t-test vs control). (F) Cell migration evaluated by wound-healing assay (*P<0.05, by Student’s t-test vs control). Data represent mean ± SD from three independent experiments.