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. 2020 Sep 24;13:9455–9463. doi: 10.2147/OTT.S259853

Figure 2.

Figure 2

Exendin-4 attenuates NF-κB-dependent SDF-1 secretion. (A) Western blot analysis of the expression of p65, p-p65, IκBα, and p-IκBα in PSCs after treatment with 50 nM exendin-4. (B) Relative quantitative comparison of Western blot analysis (**P<0.01, by Student’s t-test vs control). (C) Immunofluorescence staining of NF-κB p65 in PSCs after treatment with 50 nM exendin-4. Representative images are shown at 600× magnification. Scale bar: 20 μm. (D) qRT-PCR analysis of SDF-1 expression in PSCs treated with 50 nM exendin-4 or 50 μM of the NF-κB inhibitor PDTC (**P<0.01, by Student’s t-test vs control). (E) SDF-1 content in medium assessed by ELISA (**P<0.01, by Student’s t-test vs control). Data represent mean ± SD from three independent experiments.