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. 2020 Sep 24;13:9513–9523. doi: 10.2147/OTT.S262873

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© 2020 Wang and Lu.

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Identification of OXSM as direct target of hsa-miR338-3p. (A) hsa-miR195-5p, hsa-miR15b-5p and hsa-miR338-3p were overexpressed in the U87 cell lines by transfecting miRNA mimics; (B) the expression of OXSM in the U87 cell lines after transfecting of miRNA mimics; (C) hsa-miR338-3p were overexpressed in the U251 cell lines by transfecting miRNA mimics and the expression of OXSM in the U251 cell lines after transfecting of miRNA mimics; (D) the two putative binding sites between OXSM 3′UTR and hsa-miR338-3p; Luciferase report vectors carrying the full OXSM 3′UTR of mutation site1 (MUT-1, 437–445) and mutation site 2 (MUT-2, 358–368); (E) luminescence in hsa-miR338-3p-treated cells with wild-type reporters; (F) luminescence in hsa-miR338-3p-treated cells with MUT-1and MUT-2 reporters. *P < 0.05.