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. 2020 Sep 15;11:561948. doi: 10.3389/fimmu.2020.561948

Figure 6.

Figure 6

GBP2-dependent high mortality in Gate-16−/−Gabarap−/− mice during sepsis. (A) Wild-type (WT: n = 11), Gate-16−/−Gabarap−/− (n = 12), and Gate-16−/−Gabarap−/− Gbp2−/− (n = 9) mice were i.p. injected with 10 mg/kg body weight of poly(I:C) and then 7 h later i.p. injected with LPS (0.1 mg/kg body weight). Morbidity and mortality were observed for 48 h at 6 h intervals. (B,C) Wild-type (WT), Gate-16−/−Gabarap−/−, and Gate-16−/−Gabarap−/−Gbp2−/− mice were i.p. injected with 10 mg/kg body weight of poly(I:C) and then 7 h later i.p. injected with LPS (0.1 mg/kg body weight). Sera were taken 3 h after LPS injection from WT (n = 13), Gate-16−/−Gabarap−/− (n = 8) or Gate-16−/−Gabarap−/−Gbp2−/− (n = 9) mice for IL-1β (B) and IL-18 (C) serum concentrations were measured by ELISA. (D) CLP was performed in wild-type (WT: n = 13), Gate-16−/−Gabarap−/− (n = 13), and Gate-16−/−Gabarap−/− Gbp2−/− (n = 8) mice. Morbidity and mortality were observed for 8 days at 1 day intervals. (E,F) CLP was performed in wild-type (WT; n = 10), Gate-16−/−Gabarap−/− (n = 9) and Gate-16−/−Gabarap−/−Gbp2−/− (n = 5) mice. Peritoneal fluids were taken 16 h after CLP from the mice for IL-1β (E) and IL-6 (F) serum concentrations were measured by ELISA. The data are combined data of more than three independent experiments (A–F). Log-rank test (A,D) and two-tailed Student t-test (B,C,E,F) *P < 0.05, **P < 0.01 and ***P < 0.001.