Figure 2.

Identification of Immunogenic Gene Subtypes

(A) Unsupervised clustering of common DEGs among three ICI cluster groups to classify patients into three groups: gene clusters A–C. (B) Kaplan-Meier curves for the three groups of patients. The log rank test showed an overall p = 0.0065. (C and D) Gene Ontology (GO) enrichment analysis of the two ICI-relevant signature genes: ICI signature genes A (C) and B (D). The x axis indicates the number of genes within each GO term. (E) The fraction of tumor-infiltrating immune cells in three gene clusters. We also plotted the immune and stromal scores of three ICI clusters. The statistical difference of three ICI clusters was compared through the Kruskal-Wallis test. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001. (F and G) Difference in PD-L1 (F) and PD1 (G) expression among distinct ICI gene clusters (Kruskal-Wallis test, p < 0.0001).