FIGURE 3.

Effects of Insulin on Glucose uptake in CADASIL VSMCs. The VSMCs (CADASIL, control and UAVSMCs) were cultured and 200 μM 2-NBDG; a fluorescent indicator for monitoring glucose uptake, was added to the cells (M&M), incubated with and without 50 mM insulin. The 2-NBDG uptake was measured by flow cytometry. Flow cytometry analysis showed a reduced glucose uptake before addition of insulin in CADASIL VSMCs (*p < 0.05), however, lower glucose uptake was not affected by insulin in CADASIL VSMCs. Ctrl: control VSMC, CAD: CADASIL, MFI: mean fluorescence intensity, UAVSMC: Umbilical artery smooth muscle cells.