Fig. 3.

Apoptosis studies of on PPCL-46 cells exposed to GemHCl and 4NSG at different concentrations was performed by staining with acridine orange/ethidium bromide in GemHCl and 4NSG (fluorescence microscopy magnification of 20×). At higher concentrations (40 μM and 80 μM), 4NSG treated PPCL-46 cells showed signs of early apoptosis illustrated as greenish yellow florescence compared with GemHCl (merged images). Late apoptosis was identified as reddish-orange fluorescence in 4NSG treated cells compared with the control or GemHCl treated cells at Gem concentrations at 20 and 40 μM. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)