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. 2020 Sep 24;12:8951–8964. doi: 10.2147/CMAR.S252679

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© 2020 Zhang et al.

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Circ_CDR1as is a stable circRNA. (A) QRT-PCR assay for the relative expression of circ_CDR1as in THLE-2, Hep3B and Huh7 cells. (B and C) QRT-PCR assay for the relative expression of circ_CDR1as and GAPDH mRNA in Hep3B and Huh7 cells treated with RNase R or not. (D and E) QRT-PCR assay for the relative expression of circ_CDR1as and GAPDH mRNA in Hep3B and Huh7 cells disposed with Actinomycin D or not. (F and G) Nuclear-cytoplasmic fractionation assay for the relative expression of GAPDH, U6 and circ_CDR1as in the cytoplasmic and nuclear fractionations of Hep3B and Huh7 cells. *P < 0.05.