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. 2020 Sep 24;12:8951–8964. doi: 10.2147/CMAR.S252679

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© 2020 Zhang et al.

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Circ_CDR1as positively regulates HCC cell proliferation and metastasis. Hep3B and Huh7 cells are transfected with si-NC, si-circ_CDR1as, Vector or circ_CDR1as. (A) QRT-PCR assay for the relative expression of circ_CDR1as in transfected cells. (B and E) Colony formation assay for the number of the colonies formed by transfected cells. (C, D, F and G) MTT assay for the OD value of transfected cells at 490 nm. (H–K) Transwell assay for the migration and invasion abilities of transfected cells. (L and M) Western blot analysis for the protein levels of snail and E-cadherin in transfected cells. *P < 0.05.