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. 2020 Sep 24;12:8951–8964. doi: 10.2147/CMAR.S252679

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© 2020 Zhang et al.

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Raf1 depletion inhibits HCC cell proliferation and metastasis. (A and B) QRT-PCR and Western blot assays for the relative expression of Raf1 in THLE-2, Hep3B and Huh7 cells. (C–J) Hep3B and Huh7 cells are introduced with si-NC or si-Raf1. (C and D) QRT-PCR and Western blot assays for the relative expression of Raf1 in treated cells. (E) Colony formation assay for the number of the colonies formed by treated cells. (F and G) MTT assay for the OD value of treated cells at 490 nm. (H and I) Transwell assay for the migration and invasion abilities of treated cells. (J) Western blot analysis for the protein levels of snail and E-cadherin in treated cells. *P < 0.05.