Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 24;12:8951–8964. doi: 10.2147/CMAR.S252679

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Zhang et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

Circ_CDR1as depletion represses HCC cell proliferation and metastasis by sponging miR-1287 to downregulate Raf1. Hep3B and Huh7 cells are introduced with si-NC, si-circ_CDR1as, si-circ_CDR1as+anti-miR-NC, si-circ_CDR1as+anti-miR-1287, si-circ_CDR1as+pcDNA or si-circ_CDR1as+Raf1. (A and B) QRT-PCR and Western blot assays for the relative expression of Raf1 in treated cells. (C) Colony formation assay for the number of the colonies formed by treated cells. (D and E) MTT assay for the OD value of treated cells at 490 nm. (F and G) Transwell assay for the migration and invasion abilities of treated cells. (H) Western blot analysis for the protein levels of snail and E-cadherin in treated cells. *P < 0.05.