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. 2020 Sep 15;11:574851. doi: 10.3389/fphys.2020.574851

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Copyright © 2020 Li, Wang, Liu, Lu, Zhou, Wang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ovaries development of H. axyridis. Dissecting the ovaries of female H. axyridis in saline, after the wings were cut off, the back of the ladybird was gently made open. Remove non-reproductive tissues and organs such as fat body and digestive tract and intact ovaries could be seen at the end of the abdomen. (A) The ovarian anatomy of H. axyridis on the 3rd day after feeding. (B) The ovarian anatomy of H. axyridis on the 5th day after feeding. (C) The ovarian anatomy of the H. axyridis after 7th day after feeding.