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. 2020 Sep 15;11:561306. doi: 10.3389/fphar.2020.561306

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Copyright © 2020 Xiao, Chen, Jiang, Chen, Chen, Zhu, Zhang, Huang, Qin, Zhang, Yi and Luo

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ubc9 overexpression suppresses OGD-induced cardiomyocyte damage, and Ubc9 silencing exacerbates cardiomyocyte apoptosis under OGD treatment NRCMs were treated with Adv-Ubc9 or Adv-GFP for 12 h, and then cultured for another 36 h in fresh DMEM. After that, OGD was applied for 6 h. Alternately, short interfering RNA targeting Ubc9 or negative control siRNA was transfected in NRCMs after 48 h; the cells were in hypoxia status for 6 h. (A, D) Western blotting analysis of Ubc9 and Cleaved caspase3, n = 3–4 per group. (B, E) Representative images and quantitative analysis of TUNEL-positive cells (red) and cell nucleus (DAPI, blue), n = 5 per group. (C, F) Representative images and quantitative analysis of flow cytometry, n = 3–5 per group. Data were analyzed by one-way ANOVA, followed by a Bonferroni post-hoc test, or analyzed by unpaired Student’s t test.