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. 2020 Sep 15;11:561306. doi: 10.3389/fphar.2020.561306

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Copyright © 2020 Xiao, Chen, Jiang, Chen, Chen, Zhu, Zhang, Huang, Qin, Zhang, Yi and Luo

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Energetic autophagy flux mediates the protective effects of Ubc9 on cardiomyocytes under OGD Adv-Ubc9 or Adv-GFP was transfected in NRCMs for 36 h, and then co-treated with 3-MA (5 mM) or CQ (20 μM) for another 12 h. After that, OGD was applied for 6 h, co-treating with 3-MA (5 mM) or CQ (20 μM) for 6 h. (A, B) Western blotting analysis of LC3 Ⅱ, p62, and Cleaved caspase3, *P < 0.05, **P < 0.01, ***P < 0.001 versus corresponding group, n = 3–7 per group. (C) Representative images and quantitative analysis of TUNEL-positive cells (red) and cell nucleus (DAPI, blue), n = 4 per group. (D) Representative images and quantitative analysis of flow cytometry, n = 4 per group. Data were analyzed by one-way ANOVA, followed by a Bonferroni post-hoc test.