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. 2020 Sep 15;11:536771. doi: 10.3389/fgene.2020.536771

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Copyright © 2020 Shi, Wang, Zhang, Yuan, Chen, Chen, Chen and Cui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bimolecular fluorescence complementation assays demonstrating SMV P3 and GmGLU interaction in cytomembrane and epidermal cells of N. benthamiana. (A) SMV P3 and GmGLU were fused to N- and C-terminal of yellow fluorescent protein (YFP) respectively. YFP fluorescence in N. benthamiana leaves agroinfiltrated with pEarleyGate202-YN-GmGLU+pEarleyGate201-YC-P3. (B–D) Negative controls in the BiFC assay of GmGLU and SMV P3 interaction. Bar indicates 36 μm. (E) Fluorescence of YFP fused to GmGLU protein of host in N. benthamiana leaves under spectral confocal laser microscope. Bar indicates 140 μm. (F) Co-expression of plasmodesmata (PD) marker fused with cyan fluorescent protein (CFP) and GmGLU protein fused with YFP. GmGLU protein accumulated in dots on cytomembrane (PD marker sites). Bar indicates 20 μm.