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. 2020 Sep 15;10:554554. doi: 10.3389/fonc.2020.554554

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Copyright © 2020 Lozar, Jesenko, Kloboves Prevodnik, Cemazar, Hosta, Jericevic, Nolde and Grasic Kuhar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MCF7 cells before and after isolation using MACS technology, ×60. (A) Giemsa stain, before isolation. (B) Giemsa stain, after isolation. MCF7 cells vary in size and shape, and have prominent nucleoli (arrow). Bare nuclei and partially degenerated/apoptotic white blood cells (WBC) and red blood cells (RBC) are in the background. (C) Positive cytokeratin (CK) stain, before isolation. (D) Positive CK stain, after isolation. MCF7 cells are clearly CK positive, surrounded by CK negative WBC and RBC.