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. 2020 Sep 15;10:554554. doi: 10.3389/fonc.2020.554554

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Copyright © 2020 Lozar, Jesenko, Kloboves Prevodnik, Cemazar, Hosta, Jericevic, Nolde and Grasic Kuhar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Canonical [Patient 43, (A,B)] and non-canonical [Patient 39, (C,D)] circulating tumor cells (CTCs), ×60. (A) A morphologically preserved cell with features suggestive for CTC, Giemsa stain. (B) Positive immunocytochemical staining for cytokeratin (CK) on a Papanicolaou stained cytospin. (C) A cell resembling a lymphoid cell (arrow). Its diameter is three times the size of the surrounding erythrocytes (dotted arrow). The nucleus is ovoid with altered chromatin structure and high N/C ratio. Giemsa stain. (D) Cells exhibiting negative staining for CK and CD45 (arrow).