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. 2020 Sep 17;9(9):491–501. doi: 10.1089/wound.2019.0944

Figure 3.

Figure 3.

Blocking of NM-IIA decreases the TGF-β-mediated fibroblast contraction. (A) NF- or KF-populated collagen lattice was treated with blebbistatin (40 μM) for 24 h followed by treatment with TGF-β (20 ng/mL) for 24 h. The image of collagen lattice was taken to visualize the shrinkage of the collagen. (C) The si-NM-IIA-transfected KF-populated collagen lattice was treated with TGF-β (20 ng/mL) for 24 h. The contraction ratio of blebbistatin-treated (B) or si-NM-IIA-transfected KF (D) was shown. The contraction ratio indicated that area of collagen lattice normalized to area of each well. The experiments were repeated for at least three times to reach consistency and each experiment was done in triplicates. Color images are available online.