Figure 5.

CFAV-EVE1 Ablation Results in Increased CFAV RNA Levels upon Viral Infection

Relative CFAV RNA levels (normalized by the rp49 housekeeping gene) in heads and ovaries of the CFAV-EVE1 (+/+) (black boxplot) and CFAV-EVE1 (−/−) (white boxplot) Ae. aegypti lines on day 4 (A) and day 7 (B) post-CFAV-inoculation. Data are shown for six separate experiments represented by color- and symbol-coded data points. Relative viral RNA loads are represented by boxplots in which the box denotes the median and interquartile range (IQR) and the whiskers extend to the highest and lowest outliers within 1.5 times the IQR from the upper and lower quartiles, respectively. Multivariate analysis of variance (MANOVA) was performed for each time point and tissue separately, accounting for the experiment, mosquito line, and interaction effects. Stars indicate statistical significance of the mosquito line main effect accounting for the experiment effect (^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001; ns, not significant). The full MANOVA results are provided in Table S4. See also Table S1.