Table 1.
Details of primers used in this study.
| Genes | Primer sequence forward/reverse (5′–3′) | Tm (°C) | Length (bp) | Efficiency (%) | R2 |
|---|---|---|---|---|---|
| ACT | TCGTGTTGCCCCTGAAGAACACCCCGT | 68.8 | 129 | 107.14 | 0.9911 |
| /TGGATGGCGACGTACATAGCGGGCA | 67.3 | ||||
| GAPDH | ACCCTGAGGAGATCCCATGGGGTGA | 66.34 | 107 | 97.45 | 0.9998 |
| /TGCACCACCCTTCAAGTGAGCAGCA | 65.79 | ||||
| TUA | ACCAGTGCCTCCACCAACAGCA | 64.55 | 145 | 101.6 | 0.9944 |
| /TGCCGCCAATAACTTTGCCAGAGGA | 63.48 | ||||
| TUB | AGCTCAGGAACAGTGAGGGCCCTGT | 67.05 | 155 | 101.89 | 0.9762 |
| /TGCTGCCTTCGATTCCCTGGTCA | 63.64 | ||||
| CYP | TGGGCCAAATACGAACGGGTCCCA | 65.82 | 162 | 105.56 | 0.9975 |
| /ACAACAGGCTGCGAAGTCCTCCCA | 65.67 | ||||
| UBC | ACCCACCAACTTCATGCAGTGCAGGT | 65.47 | 106 | 98.72 | 0.9815 |
| /ACGAAAACACCTCCGGCGTAGGGGCTA | 68.2 | ||||
| His | AGGAGGCGTCGAGATTGGCGAGGTA | 66.5 | 123 | 93.12 | 0.9977 |
| /TGGTCCCCTCAGAAACAGCGTGCT | 65.67 | ||||
| PP2A | TGGACAGGCAGATCGCGCACTT | 64.55 | 127 | 109.07 | 0.9861 |
| /ACCGGACACTTGACTGGCTGCACGT | 67.77 | ||||
| HSP | TTGTCTGCCTCGGCTCTCTTCCGCA | 67.25 | 177 | 102.4 | 0.9902 |
| /TCCTTGCTGCTTGGTGACCGGA | 64.23 | ||||
| EF1-α | ACGCTCACGCTCGGCCTTAAGCTT | 66.03 | 176 | 97.9 | 0.9855 |
| /TGGTCATTGGCCACGTCGACTCTGG | 65.99 | ||||
| NtCER7 | ACCCGACTCACCACGAGGAAGCTGT | 66.87 | 149 | 96.66 | 0.9885 |
| /GGTCCTTGACGCAAGCCGCAAGCAT | 67.41 |