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. 2020 Sep 28;10:15921. doi: 10.1038/s41598-020-73092-2

Figure 5.

Figure 5

ROS/JNK signaling activation mediated APE-induced vimentin downregulation and vimentin network organization. (a) Representative confocal images of vimentin filament network in MDA-MB-231 cells treated or not (ctrl) with 200 μM EqC APE for 24 h, with or without 1 h pretreatment with 5 mM NAC and/or 5 μM SP600125. Nuclei were stained with DAPI (blue) while vimentin antibody (green) was used as cytoskeleton marker. (b) Western blot analysis of vimentin level in MDA-MB-231 cells treated with APE 200 μM EqC for 48 h with or without 1 h pretreatment with 5 mM NAC and/or 5 μM SP600125. The full-length blots are included in Supplementary Information (Fig. S6). All results were obtained from at least three independent experiments.