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. 2020 Sep 10;23(10):101549. doi: 10.1016/j.isci.2020.101549

Figure 5.

Figure 5

Identifying Genes that may Contribute to CNS Demyelination in WT Mice

Effects of viral IL-2 on gene expression in the brain of HSV-1-infected WT mice were determined on day 14 PI. RNA isolated from brains of WT mice infected with HSV-IL-2 or parental virus was used to measure the expression of Pdcd1 (PD1), CSF2 (GM-CSF), IL-5, IL-6, IFNgr1, CCL5 (RANTES), CXCL10, Tnfrsf9 (4-1BB/CD137), HIF1α, CSF2rb, Havcr2 (Tim-3), and CTLA4 in virus-infected brains. qRT-PCR was performed using total RNA as described in the Transparent Methods. Expression of respective genes in naive WT mice was used as a baseline control to estimate relative expression of each transcript in brains of infected mice. GAPDH expression was used to normalize the relative expression of each transcript. Each point represents the mean ± SEM from three brains for each mouse strain. p values were determined using a one-way ANOVA test.

Panels: (A) PD1 transcript; (B) CSF2 (GM-CSF) transcript; (C) IL-5 transcript; (D) IL-6 transcript; (E) IFNgr1 transcript; (F) CCL5 (RANTES) transcript; (G) CXCL10 transcript; (H) Tnfrsf9 (4-1BB/CD137) transcript; (I) HIF1α transcript; (J) CSF2rb transcript; (K) Havcr2 (Tim-3) transcript; and (L) CTLA4 transcript.