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. 2020 Sep 10;23(10):101549. doi: 10.1016/j.isci.2020.101549

Figure 6.

Figure 6

Determining Genes that may Contribute to CNS Demyelination in ILC2−/− Mice

LC2-deficient mice or ILC2-deficient mice that received ILC2 from WT mice were infected with HSV1-IL-2. On day 14 PI, RNA was isolated from brains of ILC2-deficient mice or ILC2-deficient mice that received ILC2 from WT as described in Figure 3. qRT-PCR was performed using total RNA, and expression of respective genes in naive ILC2-deficient mice was used as a baseline to estimate relative expression of each transcript in brains of virus-infected mice. GAPDH expression was used to normalize the relative expression of each transcript. Each point represents the mean ± SEM from three brains for each group of mice. p value was determined using a one-way ANOVA test.

Panels: (A) PD1 transcript; (B) CSF2 (GM-CSF) transcript; (C) IL-5 transcript; (D) IL-6 transcript; (E) IFNgr1 transcript; (F) CCL5 (RANTES) transcript; (G) CXCL10 transcript; (H) Tnfrsf9 (4-1BB/CD137) transcript; (I) HIF1α transcript; (J) CSF2rb transcript; (K) Havcr2 (Tim-3) transcript; and (L) CTLA4 transcript.