Figure 1.

Panc1 and AsPC1 Cell Lines Harbor Different Clock Phenotypes

(A) Panc1 and AsPC1 cells were lentivirally transduced with a BMAL1 promoter (green) or PER2 promoter (orange)-driven luciferase construct. Bioluminescence was measured for 5 consecutive days. Depicted is one representative replicate for each condition.

(B) The 48 h time course RT-qPCR measurements of selected core-clock genes (BMAL1, PER2, and NR1D1) in Panc1 and AsPC1 WT cells.

(C) The relative mRNA levels of SMAD4 compared with AsPC1.

(D) RT-qPCR measurements after knockdown of core-clock genes (shBMAL1, shPER2, and shNR1D1) in Panc1 and AsPC1 cells.

Data are expressed as mean ± SEM, n = 3, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. See also Figures S1 and S2.