Figure 2.

Hierarchal Clustering Analysis of Metabolomic Time Series Data

(A) Heatmap of changes in metabolite concentration at each time point during and after 0, 2, and 20-Hz EPS. Hierarchical clustering was performed using Ward's method with the normalized average value of each metabolite concentration. This average value was calculated with data from three biological replicates.

See also Figure S1 and Table S1.

(B) Time course of metabolites in each cluster. Dashed lines indicate the time series of each metabolite. Black, red, and blue lines indicate values during and after 0, 2, and 20-Hz EPS, respectively. Bold line represents the average value at each condition in each cluster.