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. 2020 Sep 12;23(10):101559. doi: 10.1016/j.isci.2020.101559

Figure 4.

Figure 4

LC/ESI-MS/MS Analysis of 7α-OH-Preg and 7α-OH-DHEA in Mouse Hippocampal Extract

MRM chromatograms of LC-ESI-MS/MS analysis of standard 7α-OH-Preg (A) and 7α-OH-DHEA (D) solutions (100 pg each) and the hippocampal extract prepared from two naive mice (B and E) and two trained mice (C and F). The hippocampi were isolated from the mice 2 h after the one-day training (4 trials).

(A–C) MRM transitions for detecting 7α-OH-Preg were 339.2→321.3 (claret), 339.2→303.3 (orange), and 339.2→265.2 (yellow).

(D–F) MRM transitions for detecting 7α-OH-DHEA were 311.1→293.2 (gray), 311.1→275.2 (red), and 311.1→237.2 (blue). Relative peak intensities of these product ions are shown in the inset table.