Fig. 2.

Effects of diamide on hRyR2^WT-mediated Ca²⁺waves and ER Ca²⁺load. A, representative recordings of Ca²⁺ waves from two different cells (top and bottom) expressing hRyR2^WT and SERCA2a in the control conditions (left) and during 25 μM or 50 μM diamide application (right). The dashed lines indicate the level of ER Ca²⁺ load. B, the effect of diamide on ER Ca²⁺ load. ER Ca²⁺ load was measured between Ca²⁺ waves. On average, changes in ER Ca²⁺ load induced by 25 μM (n = 22 cells) and 50 μM (n = 17 cells) diamide were not significantly different (p = 0.82; NS).