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. 2020 Sep 28;10:15859. doi: 10.1038/s41598-020-72816-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Beta diversity of the fecal bacterial community in pigs fed with very low protein diets supplemented with branched-chain amino acids. Non-metric multidimensional scaling (NMDS) of fecal microbiota for (A) CON vs. LP + BCAA, (B) LP vs. LP + BCAA, (C) CON vs. LP, (D) CON vs. LP vs. LP + BCAA. Pigs are grouped based on their dietary treatments, i.e. CON, control diet; LP, low protein diet; LP + BCAA, low protein diet supplemented with branched-chain amino acids. Each node represents an individual pig. Differences were considered significant at P < 0.05. The Permutational Multivariate Analysis of Variance (PERMANOVA) P values for CON vs. LP + BCAA, LP vs. LP + BCAA, CON vs. LP and CON vs. LP vs. LP + BCAA were < 0.004, < 0.197, 0.178 and < 0.016 respectively. n = 8.