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. 2020 Sep 30;25(3):152–163. doi: 10.15430/JCP.2020.25.3.152

Figure 5. A critical role of the cyclopentenone ring in 15d-PGJ2-induced EMT in MCF-7 cells.

Figure 5

(A) 15d-PGJ2 contains a cyclopentenone ring structure with an electrophilic α,β-unsaturated carbonyl moiety, which is absent in 9,10-dihydro-15d-PGJ2. (B, C) MCF-7 cells were treated with the indicated concentrations of 15d-PGJ2 or 9,10-dihydro-15d-PGJ2. Cells were incubated for 12 hours and 24 hours to measure the mRNA (B) and protein (C) levels of Snail and/or E-cadherin, respectively. (D) MCF-7 cells were treated with the indicated concentrations of 15d-PGJ2 or 9,10-dihydro-15d-PGJ2 for 24 hours (× 100). Scattering morphology was assessed by the cell-scattering assay. 15d-PGJ2, 15-deoxy-Δ12,14-prostaglandin J2; 9,10-dh-PGJ2, 9,10-dihydro-15d-Δ12,14-prostaglandin J2; EMT, epithelial-to-mesenchymal transition; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. aIt denotes an electrophilic carbon center.