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. 2020 Sep 25;11:417. doi: 10.1186/s13287-020-01919-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Histological analysis of injured hearts with GCaMP3+ hESC-CM grafts. a Representative cryoinjured heart with GCaMP3+ ESI-17 hESC-CM graft immunostained for beta myosin heavy chain (β-MHC) (muscle, red) and human-specific Ku80 nuclear protein (graft nuclei, brown) and counterstained with aniline blue (scar tissue, blue). Graft tissue within the box is shown at higher magnification to the right. b Graft size by histomorphometry, expressed as the percentage of injury area occupied by graft. n = 3–5 hearts per condition. c Confocal photomicrograph of cryoinjured heart with GCaMP3+ H7 hESC-CM graft near the border zone, immmunostained for GCaMP3+ (anti-GFP antibody, green) and β-myosin heavy chain (β-MHC, red). d Adjacent histological section stained with Masson’s trichrome stain. e Confocal photomicrograph of GCaMP3+ H7 hESC-CM graft near border zone immunostained for GCaMP3+ (anti-GFP antibody, green) and Cx43 (red). While grafts consistently exhibited a lower level of Cx43 expression than host myocardium, occasional scattered Cx43 gap junction plaques were observed (white arrowheads). f Adjacent histological section immunostained for GCaMP3+ and N-cadherin (red). Qualitatively similar patterns of Cx43 and N-cadherin expression were observed by grafts formed by ESI-17 and RUES2 hESC-CMs, as well as grafts with or without GCaMP expression (data not shown)