Figure 2. Blockage of the mRNA Channel in the 40S by the Nsp1 C-terminal Helical Hairpin.

(A) Cryo-EM density of the Nsp1–40S complex. Nsp1 is in cyan. Subunits of 40S that interact with Nsp1 are color-coded.

(B) Ribbon diagram of the Nsp1–40S complex. Nsp1 (cyan) binds at the mRNA channel in the cleft between the head and body of the 40S ribosomal subunit. Subunits of 40S that interact with Nsp1 are color-coded.

(C) Ribbon diagram and cryo-EM density of SARS-CoV-2 Nsp1 with schematics highlighting its interfaces to 40S.

(D) Electrostatic surface representations of Nsp1 binding surfaces to the 40S subunits and rRNA. Buried surface areas are marked.

(E) Detailed interactions between Nsp1 and the 40S ribosomal subunit.

See also Supplemental Figure 1