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. 2020 Sep 29;12:60. doi: 10.1186/s13102-020-00208-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Decomposition of the tetanic contraction with the force decrease into twitch-shape responses to successive stimuli. a, a single fast resistant motor unit force recording with a low–high–low stimulation frequency pattern. b, recording of the same motor unit at a low–high frequency stimulation. c, mathematical difference between (a) and (b), taken for the decomposition. d, a train of twitch-shape responses to successive stimuli resulting from the mathematical decomposition of recording (c). e, all decomposed twitches presented superimposed with the stimulus time accepted as 0 for all twitches. The contractile parameters of successive twitches for 10 studied motor units (mean ± SD) presented in relation to the first decomposed twitch (100%): f – twitch force, g – contraction time and (h) – half-relaxation time (mean values and standard deviations). Due to some differences between individual motor units regarding the number of stimuli at the last low-frequency phase (minimum 12), the data are presented for 12 twitches