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. 2020 Sep 29;20:469. doi: 10.1186/s12935-020-01528-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — MDM2 restoration rescues inhibitory effect of miR-585 on human glioma cell proliferation. a–c U251 cells stably infected with LV-vec or LV-miR-585 were transfected with pcDNA3.1 vector or pcDNA3.1 MDM2. a The protein expression of MDM2 was determined by Western blot analysis. β-Actin was used as a loading control. The representative images from 3 independent experiments are shown. b The cell number of U251 cells cultured for consecutive 3 and 6 days was counted by CCK-8 assay. Data are mean ± SD (n = 5). One-way ANOVA followed by post hoc Bonferroni test. **P < 0.01; *P < 0.05. c The proliferating U251 cells were stained with EdU. The percentage of EdU positive cells was analyzed by FACS assay. Data are mean ± SD (n = 5). Two-tailed unpaired Student’s t-test. **P < 0.01; *P < 0.05. d–f A172 cells stably infected with LV-vec or LV-miR-585 were transfected with pcDNA3.1 vector or pcDNA3.1 MDM2. The protein expression of MDM2 (d), cell number of A172 cells as assayed by CCK-8 (e) and EdU incorporation (f) were analyzed as in (a–c)