Fig. 7.

SB-3CT significantly reduces melanoma lung metastasis in combination with checkpoint inhibition. a Spearman’s correlation between MMP2/9 score and epithelial-to-mesenchymal transition (EMT) score. b Heatmap shows the relative expression of mesenchymal genes differentially expressed between B16F10 tumor with vehicle (n = 4) and SB-3CT treatment (n = 4). c EMT score between SB-3CT treatment and vehicle group. p value is determined by two-sided Student’s t test. d Representative images and e quantification of lung metastasis in wild-type C57/BL6 mice with B16F10 cells intravenously treated with isotype, SB-3CT, anti-PD-1, or combination strategy. f Kaplan–Meier survival curve for mice over time. g Representative images and h quantification of lung metastasis in wild-type C57/BL6 mice with B16F10 cells intravenously treated with isotype, SB-3CT, anti-PD-1, or combination strategy. i Kaplan–Meier survival curve for mice over time. Sample size per group in one experiment is 5. Error bars represent s.d. of individual mice per group in one experiment. NS, p > 0.05, *p < 0.05, **p < 0.01, and ***p < 0.001, as determined by b, c two-way ANOVA, f, i two-sided log-rank test, and e, h one-way ANOVA and Dunnett’s multiple comparison test