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Confirmation and differentiation of neural stem cells (NSCs). a Primary cultured hippocampal NSCs observed under microscope. Scale bar = 100 μm, 50 μm. b Immunofluorescence images of NSC marker nestin (red) and Sex-determining region Y box 2 (SOX2) (green). Scale bar = 20 μm. c Immunofluorescence images of β-tubulin III (red)and nestin (green). Scale bar = 100 μm. d Immunofluorescence images of glial fibrillary acidic protein (GFAP) (green) and nestin (red). Scale bar = 100 μm
