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. 2020 Sep 29;11:423. doi: 10.1186/s13287-020-01936-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — ATN1 overexpression alleviated sevoflurane-induced early NSC differentiation. a Western blotting images of β-tubulin III, GFAP, nestin, and atrophin 1(ATN1). b RT-qPCR analysis of ATN1 mRNA expression. c Immunofluorescence images of β-tubulin III (green). Scale bar = 100 μm. d Immunofluorescence images of GFAP (green). Scale bar = 100 μm. e Quantitative analysis of ATN1. f Quantitative analysis of β-tubulin III. g Quantitative analysis of GFAP. h Quantitative analysis of nestin. i Quantitative analysis of β-tubulin III on day 28. j Quantitative analysis of GFAP on day 28. k Quantification of β-tubulin III-positive cells. l Quantification of GFAP-positive cells