Table 2.
mRNA expression of seven genes in primary hCAECs treated with sera from 106 non-CBPs and 82 CBPsa
| Gene | Non-CBP | CBP | Estimate (95%CI) | RQ (95%CI)b | P |
|---|---|---|---|---|---|
| Biosensor PC1 | 0.62 (− 0.27–1.60) | − 0.26 (− 0.92–0.17) | − 0.92 (− 1.31 – − 0.53) | 1.89 (1.44–2.48) | < 0.0001 |
| CCL2 | −7.10 (−8.05 – − 6.35) | −7.67 (− 8.47 – − 7.15) | − 0.57 (− 1.00 – − 0.13) | 1.48 (1.09–2.00) | 0.011 |
| CCL5 | − 0.76 (− 1.27 – − 0.28) | −1.29 (− 1.94 – − 0.74) | − 0.48 (− 0.77 – − 0.19) | 1.39 (1.14–1.71) | 0.0012 |
| CXCL8 | −7.05 (− 9.33 – −5.10) | − 10.29 (− 12.11 – − 8.26) | −3.18 (− 4.01 – − 2.35) | 9.06 (5.10–16.11) | < 0.0001 |
| CXCL12 | − 2.63 (− 3.30 – − 2.21) | −2.33 (− 2.65 – − 1.99) | 0.62 (0.23–1.01) | 0.65 (0.50–0.85) | 0.0018 |
| ICAM | −4.56 (− 5.96 – − 3.83) | −4.96 (− 5.77 – − 4.42) | − 0.39 (− 0.72 – − 0.06) | 1.31 (1.04–1.65) | 0.021 |
| SELP | 0.07 (− 0.32–0.46) | − 0.02 (− 0.32–0.37) | − 0.15 (− 0.47–0.18) | 1.11 (0.88–1.39) | 0.37 |
| VCAM | −1.71 (− 2.66 – − 0.94) | −2.59 (− 3.15 – − 1.84) | − 0.69 (− 1.14 – − 0.24) | 1.61 (1.18–2.20) | 0.0027 |
CBP Carbon black packer, RQ Relative quantification, CI Confidence interval, hCAEC Human coronary artery endothelial cell, GLM Generalized linear model
aBiosensor PC1was the major principal component extracted from delta Cts of the seven genes. Delta Cts of target genes relative to endogenous control gene (TBP) were expressed as median and interquartile range. Estimates were calculated based on biosensor PC1 or delta Ct of individual genes using GLM with adjustment for age, overweight and obesity, current smoking status, packyears, and passage of cells
bRelative quantification was calculated as RQ = 2-(estimate)