Figure 2.

Imprinted Genes Show Distinct Expression Levels in Different Cortical Cell Types

(A) Overview of genetically defined cortical cell types that were analyzed in small bulk samples at P0.

(B) Heatmap depicting relative expression levels of imprinted genes in different cortical cell types.

(C) Normalized expression levels of three imprinted genes: Impact, Meg3, and Grb10. Note the uniform (Impact) but highly variable (Meg3 and Grb10) expression levels in distinct cell types.

(D) Cell-type specificity index (bulk), with a low specificity score indicating more uniformity and a high specificity score reflecting increasing variance of cell-type-specific expression levels.

(E) Overview of expression analysis at the single-cell level in aIPC (pink), astrocytes (purple), oligodendrocytes (orange), NI (gray), and NII (black).

(F) Heatmap depicting expression of 20 (informative) imprinted genes in distinct cell types.

(G) Expression levels of Impact, Meg3, and Grb10 in single cells.

(H) Cell-type specificity index (single cell) based on the highest fraction of cumulative expression.