Figure 5.
Neocortical Astrocytes with chr7 patUPD Are Relatively Increased to chr7 matUPD
(A and B) Analysis of brain lipid-binding protein (BLBP) (white) expression in developing cortical plate in MADM-7GT/TG;Emx1-Cre+/− at P0. matUPD cells are labeled in red (tdT+), patUPD cells are labeled in green (GFP+), and nuclei are labeled in blue (A) or unlabeled (B).
(C) Quantification of the fraction (%) of BLBP+/MADM+ double-positive cells of the total number of MADM-labeled cells with matUPD (orange) and patUPD (green).
(D–F) Analysis of the MADM-labeling pattern (cross section hemisphere (D) and boxed area (E)) and relative abundance (F) of matUPD (red, tdT+) and patUPD (green, GFP+) in CX in P21 MADM-7GT/TG;Emx1-Cre+/− mice.
(G) Quantification of the fraction (%) of MADM-labeled astrocytes of the total number of MADM-labeled cells with matUPD (orange) and patUPD (green) at P7, P14, P21, and 3 months.
(H) Quantification of the fraction (%) of Ki67+/MADM+ astrocytes of the total MADM-labeled astrocytes with matUPD (orange) and patUPD (green).
(I) Quantification of the fraction (%) of EdU+/MADM+ astrocytes of the total MADM-labeled astros with matUPD (orange) and patUPD (green).
Bars and error bars represent mean ± SEM. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001 t test. Cortical layers are indicated (roman numerals). Scale bar: 50 μm (A and B), 25 μm (b′–b″′, h′, h″, i′, and i″), 500 μm (D), and 60 μm (E). See also Figure S6.