Extended Data Fig. 8. Effects of acivicin in LPS-induced neurodegeneration.

a, GGT activity in substantia nigra (SN) extracts 12 h following PBS or LPS injection in mice pretreated with acivicin or saline for 5 d. Data are from n = 6 mice (control, PBS, or LPS + saline) or n = 8 mice (LPS + acivicin). b, c, Microscopy images of brain sections spanning SN 7 d following PBS or LPS injection in mice pretreated with acivicin or saline, tissues were stained for tyrosine-hydroxylase (TH) (b) or Iba-1 (c). Scale bars, 300 μm (b, c). Quantification of TH (b) and Iba-1 (c) immunoreactivity in SN is shown (right). Data are from n = 6 mice (saline) and n = 5 mice (acivicin) (b); n = 6 mice (saline + PBS or saline + LPS) and n = 5 mice (acivicin + PBS or acivicin + LPS) (c).Each symbol represents an individual mouse (a-c). Data are shown as means ± s.e.m. (a-c). * P < 0.05, **P < 0.01, *** P < 0.001, as determined by two-way ANOVA with Tukey’s multiple comparisons test (a), two-tailed Mann-Whitney test (b), or two-way ANOVA with Sidak’s multiple comparisons test (c).