Fig. 1.

Elevated expression of LY6K is inversely correlates with GBM patient survival. (A) LY6K was among the top differentially expressed genes in MES-like GSCs, relative to PN-like GSCs. (B) LY6K expression correlated with poor patient survival in GBM samples in a five-year analysis of TCGA datasets. (C) Among all tested variables, only age (<60 y) correlated with poor survival in GBM patients in multivariate analyses with LY6K in TCGA datasets. (D) Expression of LY6K was higher in MES GBM samples, relative to CL or PN in TCGA datasets. (E, F) Immunoblot (IB) for LY6K expression in PN-like and MES-like GSCs (E) and GBM cell lines and HeLa cells (F). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was a loading control. Data are representative of 3 independent experiments with similar results. *P < 0.05.