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. 2020 Feb 14;22(9):1315–1326. doi: 10.1093/neuonc/noaa032

Fig. 2.

Fig. 2

LY6K enhances GBM cell proliferation, sphere-forming frequencies, and tumorigenicity. (A–C) In MES-like GSC83 and GSC30, knockdown of LY6K suppressed cell proliferation (A), sphere-forming frequencies (B), and in vivo tumor growth (C). (D–F) Rescuing LY6K in GSC83 and GSC30 with knockdown of endogenous LY6K restored cell proliferation (D), sphere-forming frequencies (E), and in vivo tumor growth and survival (F). For (F) Top, BLI. Bottom, Kaplan–Meier survival analysis. (G) Overexpression of exogenous LY6K in GSC528 increased cell proliferation (left) and sphere-forming frequencies (right). (H) Overexpression of LY6K in U87 cells increased cell proliferation. (I) Bright-field phase contrast representative images showing cell growth at day 8 of proliferation assay from (H). Scale bar in (I) is 100 μm. Insets in (A), (D), (G), (H): IB for LY6K and GAPDH (loading control) in indicated GSCs. Data are representative of 2–3 independent experiments with similar results. **P < 0.03, ***P < 0.01.