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. 2020 Sep 15;11:581011. doi: 10.3389/fphar.2020.581011

Figure 5.

Figure 5

The HCK inhibitor A419259 attenuated LPS-induced inflammatory response in vivo. (A) Schematic graph illustrating the experimental timelines. (B) ELISA of the serum cleaved IL-1β (P17) from mice intraperitoneally injected with LPS (5 mg/kg of body weight) with or without A419259 (30 mg/kg of body weight) (n = 7 for each group). (C) Representative images of liver H&E staining (three mice, nine slices each group). (D) Immunoblotting analysis of cleaved caspase 1 (P20), NLRP3, or GAPDH in the livers of mice intraperitoneally injected with LPS with or without A419259 (n = 7 for each group). (E) Analysis of the changes in NLRP3 or cleaved caspase 1 (P20) protein level. (F) qRT-PCR analysis of nlrp3, Caspase1, Il-1β, Il-18, Asc, Tnf-α, Il-6, Il-10, and Il-4 in the livers of mice intraperitoneally injected with LPS with or without A419259 (n = 7 for each group). Data show means ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001. ns, P > 0.05, Student’s t-test.