Figure 10.

Doxycycline and Doxy-Myr have no effect on tumor growth. MDA-MB-231 cells and the well-established chorio-allantoic membrane (CAM) assay in chicken eggs were used to quantitatively measure tumor growth. An inoculum of 1 × 10⁶ MDA-MB-231 cells was added onto the Upper CAM of each egg (on Day E9) and then eggs were then randomized into groups. On day E10, tumors were detectable and they were then treated daily for 8 days with vehicle alone (1% DMSO in PBS), Doxycycline or Doxy-Myr. After 8 days of drug administration, on day E18 all tumors were weighed. Note that Doxycycline and Doxy-Myr did not have any significant effects on tumor growth. Averages are shown ± SEM. NS, not significant.