Figure 3.

Swim-up capacitation time changes on sperm biomarkers. (a) Binary plot of the first two principal components (PC1 and PC2) accounting for 57.1% of total variance. The ellipses include 95% confidence regions of within-subpopulation sample variability. (b) The labeled rays show the loadings for lectin patterns onto PC1 and PC2 axes (see Table 2 for additional biomarker correlation details). (c) Glycocalyx sketch based on strong lectin correlations in different physiological conditions according to b data. T0 is composed of P4AAA, P1ConA, P1PNA, and P1WGA; T1 is composed of P2AAA, P1ConA, P1PNA, and P4WGA; T4 is composed of P1AAA, P2ConA, P1PNA, and P4WGA. AAA: Aleuria aurantia agglutinin; ConA: Concanavalin A; PNA: Peanut agglutinin; WGA: Wheat germ agglutinin.