Table 1.
Descriptive and statistical results of sperm biomarkers in uncapacitated and swim-up capacitated conditions
| Uncapacitated, mean±s.d. | 1 h capacitated, mean±s.d. | 4 h capacitated, mean±s.d. | |
|---|---|---|---|
| Concentration (106 ml-1) | 54.7±26.2 | 8.6±5.9a | 8.9±3.5b |
| Total motility (%) | 70.9±14.8 | 97.5±1.9a | 96.3±1.5b |
| Viability (%) | 82.5±15.1 | 96.7±2.3a | 95.9±1.6b |
| Normal morphology (%) | 9.7±6.2 | 19.3±9.2 | 26.6±13.8b |
| Acrosomal reaction (%) | |||
| Spontaneous | 12.0±6.7 | 16.5±9.5 | 23.8±8.2b |
| Calcium ionophore | − | 80.9±6.8 | 81.2±6.9 |
| Tyrosine phosphorylation (%) | |||
| ES | 1.5±3.2 | 16.5±6.0a | 18.1±7.2b |
| PP | 3.2±1.9 | 17.2±5.6a | 25.3±4.5b,c |
| ES + PP | 0.0±0.0 | 5.5±4.4a | 9.7±7.2b |
| DNA fragmentation (%) | 14.0±12.7 | 3.3±4.0 | 2.0±2.3b |
| Sugar-binding patterns, AAA lectin (%) | |||
| P1 | 28.7±16.2 | 43.3±20.1 | 59.3±15.4b |
| P2 | 6.0±7.3 | 32.4±17.6a | 21.1±10.6b |
| P3 | 4.0±6.6 | 2.3±6.2 | 2.2±5.7 |
| P4 | 45.2±15.1 | 4.8±5.8a | 3.6±4.8b |
| P5 | 16.0±11.8 | 17.0±18.3 | 13.6±11.1 |
| Sugar-binding patterns, ConA lectin (%) | |||
| P1 | 55.5±16.9 | 49.0±24.0 | 38.9±20.2 |
| P2 | 26.0±13.9 | 26.4±14.9 | 45.0±17.0b,c |
| P3 | 3.4±4.5 | 1.3±4.0 | 2.7±4.8 |
| P4 | 4.6±10.7 | 3.2±5.8 | 7.9±4.7 |
| P5 | 10.2±8.5 | 19.8±26.5 | 5.3±6.6 |
| Sugar-binding patterns, PNA lectin (%) | |||
| P1 | 45.8±29.9 | 67.8±21.8 | 68.0±21.6 |
| P3 | 19.9±15.9 | 9.8±10.0 | 17.9±13.3 |
| P5 | 34.2±30.9 | 22.3±22.6 | 14.0±14.0 |
| Sugar-binding patterns, WGA lectin (%) | |||
| P1 | 59.7±12.9 | 41.3±11.2a | 35.1±7.3b |
| P3 | 7.4±9.0 | 4.5±6.3 | 13.4±10.9 |
| P4 | 23.1±10.3 | 47.4±12.0a | 42.1±17.0b |
| P5 | 9.9±10.7 | 6.7±9.1 | 9.2±10.2 |
Letters a, b, and c indicate significant differences at P<0.05 level according to Kruskal–Wallis test between capacitation times: aT0 versus T1, bT0 versus T4, and cT1 versus T4. ES: equatorial segment; PP: principal piece; AAA: Aleuria aurantia agglutinin; ConA: Concanavalin A; PNA: Peanut agglutinin; WGA: Wheat germ agglutinin; s.d.: standard deviation