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. 2019 Oct 15;22(5):447–453. doi: 10.4103/aja.aja_104_19

Table 1.

Descriptive and statistical results of sperm biomarkers in uncapacitated and swim-up capacitated conditions

Uncapacitated, mean±s.d. 1 h capacitated, mean±s.d. 4 h capacitated, mean±s.d.
Concentration (106 ml-1) 54.7±26.2 8.6±5.9a 8.9±3.5b
Total motility (%) 70.9±14.8 97.5±1.9a 96.3±1.5b
Viability (%) 82.5±15.1 96.7±2.3a 95.9±1.6b
Normal morphology (%) 9.7±6.2 19.3±9.2 26.6±13.8b
Acrosomal reaction (%)
 Spontaneous 12.0±6.7 16.5±9.5 23.8±8.2b
 Calcium ionophore 80.9±6.8 81.2±6.9
Tyrosine phosphorylation (%)
 ES 1.5±3.2 16.5±6.0a 18.1±7.2b
 PP 3.2±1.9 17.2±5.6a 25.3±4.5b,c
 ES + PP 0.0±0.0 5.5±4.4a 9.7±7.2b
 DNA fragmentation (%) 14.0±12.7 3.3±4.0 2.0±2.3b
Sugar-binding patterns, AAA lectin (%)
 P1 28.7±16.2 43.3±20.1 59.3±15.4b
 P2 6.0±7.3 32.4±17.6a 21.1±10.6b
 P3 4.0±6.6 2.3±6.2 2.2±5.7
 P4 45.2±15.1 4.8±5.8a 3.6±4.8b
 P5 16.0±11.8 17.0±18.3 13.6±11.1
Sugar-binding patterns, ConA lectin (%)
 P1 55.5±16.9 49.0±24.0 38.9±20.2
 P2 26.0±13.9 26.4±14.9 45.0±17.0b,c
 P3 3.4±4.5 1.3±4.0 2.7±4.8
 P4 4.6±10.7 3.2±5.8 7.9±4.7
 P5 10.2±8.5 19.8±26.5 5.3±6.6
Sugar-binding patterns, PNA lectin (%)
 P1 45.8±29.9 67.8±21.8 68.0±21.6
 P3 19.9±15.9 9.8±10.0 17.9±13.3
 P5 34.2±30.9 22.3±22.6 14.0±14.0
Sugar-binding patterns, WGA lectin (%)
 P1 59.7±12.9 41.3±11.2a 35.1±7.3b
 P3 7.4±9.0 4.5±6.3 13.4±10.9
 P4 23.1±10.3 47.4±12.0a 42.1±17.0b
 P5 9.9±10.7 6.7±9.1 9.2±10.2

Letters a, b, and c indicate significant differences at P<0.05 level according to Kruskal–Wallis test between capacitation times: aT0 versus T1, bT0 versus T4, and cT1 versus T4. ES: equatorial segment; PP: principal piece; AAA: Aleuria aurantia agglutinin; ConA: Concanavalin A; PNA: Peanut agglutinin; WGA: Wheat germ agglutinin; s.d.: standard deviation