Figure 6.

Hart's elastin staining in rat models (n = 6 per group). (a) Tunica albuginea showed blue-black to blue elastic fibers, which was abundant between Buck's fascia and tunica (at ×50 magnification, scale bars = 200 μm), representative elastic fibers on the upper left box (at ×400 magnification, scale bars = 50 μm); elastic fibers with regular shapes were tightly intertwined with collagen bundles along tunica albuginea. (b) Representative elastic fibers of corpus spongiosum at ×100 magnification (scale bars = 100 μm). Corpus spongiosum revealed substantially abundant elastic fibers with a tortuous appearance. (c) Elastic fibers of corpus spongiosum at 0 o'clock (at ×200 magnification, scale bars = 100 μm). (d) Cumulative area of elastic fibers in corpus spongiosum (0, 3, 6, and 9 o'clock at ×200 magnification), with insignificant difference found (P = 0.1865). Anti-LOX: intragastric administration of β-aminopropionitrile (BAPN) with a dose of 100 mg kg⁻¹ day⁻¹; VD: vacuum device aspiration with negative pressure of -300 mmHg. LOX: lysyl oxidase.