FIG 1.

hAgo2 downregulated DICER1 expression. (A to D) Western blot of various proteins involved in the miRNA pathway, using samples from HeLa cells, HuH7, or A549 cells, transiently transfected with different amounts of either vector or the plasmid expressing hAgo2, as indicated (A), stably transfected with GFP or hAgo2 (two different infection experiments, 1 and 2) (B), transfected with different amounts of siRNA targeting hAgo2, as indicated (C), or stably transfected with shRNA targeting hAgo2 (two different shRNA clones, +6 and +8) (D). (E) Western blot of DICER1 and hAgo2 proteins using samples from HeLa cells (left) or A549 cells (right) transfected with sgRNA targeting hAgo2 (two different clones [2-5 and 1-5] were from HeLa cells, while the other two [2-19 and 1-14] were from A549 cells).