Extended Data Fig. 7:

Intrainfarct application of AMD3100 induces ectopic positioning of MDM after PT. Cxcr4_CreER/wt; R26_CAG-LSL-tdT mice received saline or AMD3100 into the infarct at day 8 after PT via stereotactically-controlled injection. Images were captured 6 hours after substance application. a, Counterstained tdT and GFAP. Regions of interest (ROIs) used to quantify the presence of MDM are illustrated (ROI#1: infarct, ROI#2: peri-infarct, ROI#3: intact region). b, Iba1 staining (green) is shown for cells labeled with arrows in a. DAPI appears white. The depicted tdT⁺ cells in the saline-treated animal are Iba1⁻ presumptive neurons. The tdT⁺ Iba1⁺ cell in the AMD3100-treated animal represents an ectopic MDM in ROI#3. a,b, Images are representative for n=6 each. Scale bars: 200 μm (a), 20 μm (b).