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. 2020 Sep 29;15(9):e0239870. doi: 10.1371/journal.pone.0239870

Fig 5. Changes of the chemical shifts of the backbone amide resonance between Arc280-396 and Arc208-396/Arc280-363.

Fig 5

(A) The overlay of NMR TROSY spectra of Arc280-396 (blue) and Arc208-396 (purple). (B) The chemical shift perturbations (CSP) of residues 280–396 when the amide chemical shifts of Arc208-396 and Arc280-396 are compared with each other. (C) The overlay of NMR TROSY spectra of Arc280-396 (blue) and Arc280-363 (red). (D) The chemical shift perturbations (CSP) of residues 280–363 when the amide chemical shifts of Arc280-363 and Arc280-396 are compared with each other. The CSPs of backbone amides are calculated using the equation [(ΔH)2+(ΔN)2/5]1/2, where ΔH and ΔN are the changes in the 1H and 15N chemical shifts respectively. The chemical shift data were deposited in the BMRB database (BMRB ID: 50436 (Arc280-396), 50440 (Arc208-396), and 50441 (Arc280-363)).